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Registro Completo |
Biblioteca(s): |
Embrapa Pecuária Sul; Embrapa Pesca e Aquicultura. |
Data corrente: |
16/02/2016 |
Data da última atualização: |
16/02/2016 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
BENAVIDES, M. V.; SONSTEGARD, T. S.; KEMP, S.; MUGAMBI, J. M.; GIBSON, J. P.; BAKER, R. L.; HANOTTE, O.; MARSHALL, K.; VAN TASSELL, C. |
Afiliação: |
MAGDA VIEIRA BENAVIDES, CNPASA; TAD S. SONSTEGARD, USDA; STEPHEN KEMP, ILRI; JOHN M. MUGAMBI, KENYA AGRICULTURAL RESEARCH INSTITUTE; JOHN P. GIBSON, UNIVERSITY OF NEW ENGLAND; ROBERT LEYDEN BAKER, WAIKATO, NEW ZEALAND; OLIVIER HANOTTE, UNIVERSITY OF NOTTINGHAM; KAREN MARSHALL, ILRI; CURTIS VAN TASSELL, USDA. |
Título: |
Identification of novel loci associated with gastrointestinal parasite resistance in a Red Maasai x Dorper backcross population. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Plos One, v. 10, n. 4, e0122797, Apr. 2015. |
DOI: |
10.1371/journal.pone.0122797 |
Idioma: |
Inglês |
Conteúdo: |
Gastrointestinal (GI) parasitic infection is the main health constraint for small ruminant production, causing loss of weight and/or death. Red Maasai sheep have adapted to a tropical environment where extreme parasite exposure is a constant, especially with highly pathogenic Haemonchus contortus. This breed has been reported to be resistant to gastrointestinal parasite infection, hence it is considered an invaluable resource to study associations between host genetics and resistance. The aim of this study was to identify polymorphisms strongly associated with host resistance in a double backcross population derived from Red Maasai and Dorper sheep using a SNP-based GWAS analysis. The animals that were genotyped represented the most resistant and susceptible individuals based on the tails of phenotypic distribution (10% each) for average faecal egg counts (AVFEC). AVFEC, packed cell volume (AVPCV), and live weight (AVLWT) were adjusted for fixed effects and co-variables, and an association analysis was run using EMMAX. Revised significance levels were calculated using 100,000 permutation tests. The top five significant SNP markers with - log10 p-values >3.794 were observed on five different chromosomes for AVFEC, and BLUPPf90/PostGSf90 results confirmed EMMAX significant regions for this trait. One of these regions included a cluster of significant SNP on chromosome (Chr) 6 not in linkage disequilibrium to each other. This genomic location contains annotated genes involved in cytokine signalling, haemostasis and mucus biosynthesis. Only one association detected on Chr 7 was significant for both AVPCV and AVLWT. The results generated here reveal candidate immune variants for genes involved in differential response to infection and provide additional SNP marker information that has potential to aid selection of resistance to gastrointestinal parasites in sheep of a similar genetic background to the double backcross population. MenosGastrointestinal (GI) parasitic infection is the main health constraint for small ruminant production, causing loss of weight and/or death. Red Maasai sheep have adapted to a tropical environment where extreme parasite exposure is a constant, especially with highly pathogenic Haemonchus contortus. This breed has been reported to be resistant to gastrointestinal parasite infection, hence it is considered an invaluable resource to study associations between host genetics and resistance. The aim of this study was to identify polymorphisms strongly associated with host resistance in a double backcross population derived from Red Maasai and Dorper sheep using a SNP-based GWAS analysis. The animals that were genotyped represented the most resistant and susceptible individuals based on the tails of phenotypic distribution (10% each) for average faecal egg counts (AVFEC). AVFEC, packed cell volume (AVPCV), and live weight (AVLWT) were adjusted for fixed effects and co-variables, and an association analysis was run using EMMAX. Revised significance levels were calculated using 100,000 permutation tests. The top five significant SNP markers with - log10 p-values >3.794 were observed on five different chromosomes for AVFEC, and BLUPPf90/PostGSf90 results confirmed EMMAX significant regions for this trait. One of these regions included a cluster of significant SNP on chromosome (Chr) 6 not in linkage disequilibrium to each other. This genomic location contains annotated genes involved i... Mostrar Tudo |
Thesagro: |
Doença animal; Haemonchus Contortus; Nematoide; Ovelha. |
Thesaurus Nal: |
Digestive system diseases; Sheep. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/139121/1/CNPASA-mvb.pdf
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Marc: |
LEADER 02850naa a2200301 a 4500 001 2037157 005 2016-02-16 008 2015 bl uuuu u00u1 u #d 024 7 $a10.1371/journal.pone.0122797$2DOI 100 1 $aBENAVIDES, M. V. 245 $aIdentification of novel loci associated with gastrointestinal parasite resistance in a Red Maasai x Dorper backcross population.$h[electronic resource] 260 $c2015 520 $aGastrointestinal (GI) parasitic infection is the main health constraint for small ruminant production, causing loss of weight and/or death. Red Maasai sheep have adapted to a tropical environment where extreme parasite exposure is a constant, especially with highly pathogenic Haemonchus contortus. This breed has been reported to be resistant to gastrointestinal parasite infection, hence it is considered an invaluable resource to study associations between host genetics and resistance. The aim of this study was to identify polymorphisms strongly associated with host resistance in a double backcross population derived from Red Maasai and Dorper sheep using a SNP-based GWAS analysis. The animals that were genotyped represented the most resistant and susceptible individuals based on the tails of phenotypic distribution (10% each) for average faecal egg counts (AVFEC). AVFEC, packed cell volume (AVPCV), and live weight (AVLWT) were adjusted for fixed effects and co-variables, and an association analysis was run using EMMAX. Revised significance levels were calculated using 100,000 permutation tests. The top five significant SNP markers with - log10 p-values >3.794 were observed on five different chromosomes for AVFEC, and BLUPPf90/PostGSf90 results confirmed EMMAX significant regions for this trait. One of these regions included a cluster of significant SNP on chromosome (Chr) 6 not in linkage disequilibrium to each other. This genomic location contains annotated genes involved in cytokine signalling, haemostasis and mucus biosynthesis. Only one association detected on Chr 7 was significant for both AVPCV and AVLWT. The results generated here reveal candidate immune variants for genes involved in differential response to infection and provide additional SNP marker information that has potential to aid selection of resistance to gastrointestinal parasites in sheep of a similar genetic background to the double backcross population. 650 $aDigestive system diseases 650 $aSheep 650 $aDoença animal 650 $aHaemonchus Contortus 650 $aNematoide 650 $aOvelha 700 1 $aSONSTEGARD, T. S. 700 1 $aKEMP, S. 700 1 $aMUGAMBI, J. M. 700 1 $aGIBSON, J. P. 700 1 $aBAKER, R. L. 700 1 $aHANOTTE, O. 700 1 $aMARSHALL, K. 700 1 $aVAN TASSELL, C. 773 $tPlos One$gv. 10, n. 4, e0122797, Apr. 2015.
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Registro original: |
Embrapa Pesca e Aquicultura (CNPASA) |
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Registro Completo
Biblioteca(s): |
Embrapa Solos. |
Data corrente: |
25/11/2019 |
Data da última atualização: |
25/11/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
LEMES, M. W. A.; MACHADO, A. S.; VASQUES, G. de M.; RODRIGUES, H. M.; LOPES, R. T.; ROSAS, R. O. |
Afiliação: |
MARCELO WERMELINGER AGUIAR LEMES, UNESA; ALESSANDRA SILVEIRA MACHADO, UFRJ; GUSTAVO DE MATTOS VASQUES, CNPS; HUGO MACHADO RODRIGUES, UFRRJ; RICARDO TADEU LOPES, UFRJ; REINER OLÍBANO ROSAS, UFF. |
Título: |
Influence of soil cover on pore distribution and connectivity density in a Ferralssol evaluated by 3D computerized microtomography. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Mercator, v. 18, e18029, 2019. |
DOI: |
10.4215/rm2019.e18029 |
Idioma: |
Inglês |
Notas: |
Título em português: Influência da cobertura do solo na distribuição de poros e densidade de conectividade em um Latossolo avaliado através da microtomografia computadorizada 3D.
Título em espanhol: Influencia de la cobertura del suelo en la distribución de poros y densidad de conectividad en um latosol evaluado a través de la microtomografía computarizada 3D. |
Conteúdo: |
In the search for new techniques aimed at complementing and adding new data on Ferralsols, high resolution computer microtomography (microCT) appears as a non-destructive and fast analytical technique. MicroCT has been outstanding in the international scenarios and it is more and more present in soil analyses. Analyzing a soil sample by microCT allows obtaining knowledge, in microscale, on shape, size, distribution, volume, area and pore connectivity and having a 3D visualization of the soil sample and its structure. The objective of this work is to use microCT to compare the porosity distribution and pore connectivity density between a soil without vegetation cover and a soil with grass cover, both Ferralsols. Four undisturbed samples of each soil were collected. The results show that the MicroCT technique is an efficient and non-destructive tool for the analysis and characterization of the pore structure of soils protected from and degraded by erosion, underlining clear differences between them, as expected. Na busca por novas técnicas que visem complementar e agregar novos dados sobre Latossolos, a microtomografia computadorizada de alta resolução (microCT) aparece como uma técnica analítica não destrutiva e rápida. A MicroCT tem se destacado nos cenários internacionais e está cada vez mais presente nas análises de solo. A análise de uma amostra de solo por microCT permite obter conhecimento, em microescala, da forma, tamanho, distribuição, volume, área e conectividade dos poros e ter uma visualização 3D da amostra de solo e sua estrutura. O objetivo deste trabalho é utilizar microCT para comparar a distribuição da porosidade e a densidade de conectividade do poro entre um solo sem cobertura vegetal e um solo com cobertura de grama, ambos Latossolos. Quatro amostras não perturbadas de cada área foram coletadas. Os resultados mostram que a técnica MicroCT é uma ferramenta eficiente e não destrutiva para a análise e caracterização da estrutura porosa dos solos protegidos e degradados pela erosão, evidenciando claras diferenças entre eles, conforme esperado. En la búsqueda de nuevas técnicas destinadas a complementar y agregar nuevos datos sobre Ferralsoles, la microtomografía computarizada de alta resolución (microCT) aparece como una técnica analítica no destructiva y rápida. MicroCT ha sido sobresaliente en los escenarios internacionales y está cada vez más presente en los análisis de suelos. El análisis de una muestra de suelo mediante microCT permite obtener conocimiento, a microescala, de la forma, el tamaño, la distribución, el volumen, el área y la conectividad de poros y tener una visualización 3D de la muestra de suelo y su estructura. El objetivo de este trabajo es utilizar microCT para comparar la distribución de porosidad y la densidad de conectividad de poros entre un suelo sin cubierta vegetal y un suelo con cubierta de pasto, ambos Ferralsoles. Se recogieron cuatro muestras inalteradas de cada suelo. Los resultados muestran que la técnica MicroCT es una herramienta eficiente y no destructiva para el análisis y la caracterización de la estructura de poro de los suelos protegidos y degradados por la erosión, subrayando diferencias claras entre ellos, como se esperaba. MenosIn the search for new techniques aimed at complementing and adding new data on Ferralsols, high resolution computer microtomography (microCT) appears as a non-destructive and fast analytical technique. MicroCT has been outstanding in the international scenarios and it is more and more present in soil analyses. Analyzing a soil sample by microCT allows obtaining knowledge, in microscale, on shape, size, distribution, volume, area and pore connectivity and having a 3D visualization of the soil sample and its structure. The objective of this work is to use microCT to compare the porosity distribution and pore connectivity density between a soil without vegetation cover and a soil with grass cover, both Ferralsols. Four undisturbed samples of each soil were collected. The results show that the MicroCT technique is an efficient and non-destructive tool for the analysis and characterization of the pore structure of soils protected from and degraded by erosion, underlining clear differences between them, as expected. Na busca por novas técnicas que visem complementar e agregar novos dados sobre Latossolos, a microtomografia computadorizada de alta resolução (microCT) aparece como uma técnica analítica não destrutiva e rápida. A MicroCT tem se destacado nos cenários internacionais e está cada vez mais presente nas análises de solo. A análise de uma amostra de solo por microCT permite obter conhecimento, em microescala, da forma, tamanho, distribuição, volume, área e conectividade do... Mostrar Tudo |
Palavras-Chave: |
Técnica de microtomografia. |
Thesagro: |
Estrutura do Solo; Porosidade; Solo. |
Thesaurus NAL: |
Porosity; Soil; Soil structure. |
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/205337/1/Influence-of-soil-cover-on-pore-distribution-and-connectivity-density-in-a-ferralssol-evaluated-by-3D-computerized-microtomography-2019.pdf
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Marc: |
LEADER 04490naa a2200289 a 4500 001 2114990 005 2019-11-25 008 2019 bl uuuu u00u1 u #d 024 7 $a10.4215/rm2019.e18029$2DOI 100 1 $aLEMES, M. W. A. 245 $aInfluence of soil cover on pore distribution and connectivity density in a Ferralssol evaluated by 3D computerized microtomography.$h[electronic resource] 260 $c2019 500 $aTítulo em português: Influência da cobertura do solo na distribuição de poros e densidade de conectividade em um Latossolo avaliado através da microtomografia computadorizada 3D. Título em espanhol: Influencia de la cobertura del suelo en la distribución de poros y densidad de conectividad en um latosol evaluado a través de la microtomografía computarizada 3D. 520 $aIn the search for new techniques aimed at complementing and adding new data on Ferralsols, high resolution computer microtomography (microCT) appears as a non-destructive and fast analytical technique. MicroCT has been outstanding in the international scenarios and it is more and more present in soil analyses. Analyzing a soil sample by microCT allows obtaining knowledge, in microscale, on shape, size, distribution, volume, area and pore connectivity and having a 3D visualization of the soil sample and its structure. The objective of this work is to use microCT to compare the porosity distribution and pore connectivity density between a soil without vegetation cover and a soil with grass cover, both Ferralsols. Four undisturbed samples of each soil were collected. The results show that the MicroCT technique is an efficient and non-destructive tool for the analysis and characterization of the pore structure of soils protected from and degraded by erosion, underlining clear differences between them, as expected. Na busca por novas técnicas que visem complementar e agregar novos dados sobre Latossolos, a microtomografia computadorizada de alta resolução (microCT) aparece como uma técnica analítica não destrutiva e rápida. A MicroCT tem se destacado nos cenários internacionais e está cada vez mais presente nas análises de solo. A análise de uma amostra de solo por microCT permite obter conhecimento, em microescala, da forma, tamanho, distribuição, volume, área e conectividade dos poros e ter uma visualização 3D da amostra de solo e sua estrutura. O objetivo deste trabalho é utilizar microCT para comparar a distribuição da porosidade e a densidade de conectividade do poro entre um solo sem cobertura vegetal e um solo com cobertura de grama, ambos Latossolos. Quatro amostras não perturbadas de cada área foram coletadas. Os resultados mostram que a técnica MicroCT é uma ferramenta eficiente e não destrutiva para a análise e caracterização da estrutura porosa dos solos protegidos e degradados pela erosão, evidenciando claras diferenças entre eles, conforme esperado. En la búsqueda de nuevas técnicas destinadas a complementar y agregar nuevos datos sobre Ferralsoles, la microtomografía computarizada de alta resolución (microCT) aparece como una técnica analítica no destructiva y rápida. MicroCT ha sido sobresaliente en los escenarios internacionales y está cada vez más presente en los análisis de suelos. El análisis de una muestra de suelo mediante microCT permite obtener conocimiento, a microescala, de la forma, el tamaño, la distribución, el volumen, el área y la conectividad de poros y tener una visualización 3D de la muestra de suelo y su estructura. El objetivo de este trabajo es utilizar microCT para comparar la distribución de porosidad y la densidad de conectividad de poros entre un suelo sin cubierta vegetal y un suelo con cubierta de pasto, ambos Ferralsoles. Se recogieron cuatro muestras inalteradas de cada suelo. Los resultados muestran que la técnica MicroCT es una herramienta eficiente y no destructiva para el análisis y la caracterización de la estructura de poro de los suelos protegidos y degradados por la erosión, subrayando diferencias claras entre ellos, como se esperaba. 650 $aPorosity 650 $aSoil 650 $aSoil structure 650 $aEstrutura do Solo 650 $aPorosidade 650 $aSolo 653 $aTécnica de microtomografia 700 1 $aMACHADO, A. S. 700 1 $aVASQUES, G. de M. 700 1 $aRODRIGUES, H. M. 700 1 $aLOPES, R. T. 700 1 $aROSAS, R. O. 773 $tMercator$gv. 18, e18029, 2019.
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